IMAGINE a technology that could target pesticides to treat specific spots deep within the soil, making them more effective at controlling infestations while limiting their toxicity to the environment.

Researchers at the University of California San Diego and Case Western Reserve University have taken a step toward that goal.

They discovered that a biological nanoparticle – a plant virus – is capable of delivering pesticide molecules deeper below the ground, to places that are normally beyond their reach.

The work could help farmers better manage difficult pests, like parasitic nematodes that wreak havoc on plant roots deep in the soil, with less pesticide. The work is published May 20 in the journal Nature Nanotechnology.

“It sounds counter-intuitive that we can use a plant virus to treat plant health,” UC San Diego Jacobs School of Engineering professor of nanoengineering and senior author of the study, Nicole Steinmetz, said.

“This is an emerging field of research in nanotechnology showing that we can use plant viruses as pesticide delivery systems. It’s similar to how we’re using nanoparticles in medicine to target drugs towards sites of disease and reduce their side effects in patients.”

Pesticides are very sticky molecules when applied in the field. They bind strongly to organic matter in the soil, making it difficult to get enough to penetrate deep down into the root level where pests like nematodes reside and cause damage.

To compensate, farmers end up applying large amounts of pesticides, which cause harmful residues to build up in the soil and leach into groundwater.

Steinmetz and her team are working to address this problem.

In a new study, they discovered that a particular plant virus, Tobacco mild green mosaic virus, can transport small amounts of pesticide deep through the soil with ease.

A helpful virus

In lab tests, the researchers attached a model insecticide to different types of nanoparticles and watered them through columns of soil.

Tobacco mild green mosaic virus outperformed most of the other nanoparticles tested in the study. It carried its cargo down to 30 centimetres below the surface.

PLGA and mesoporous silica nanoparticles, which researchers have studied for pesticide and fertiliser delivery, carried their payloads eight and 12cm deep, respectively.

Other plant viruses were also tested.

Cowpea mosaic virus also carried its payload 30cm deep below the surface, but it can only carry a fraction of the payload that Tobacco mild green mosaic virus can carry.

Interestingly, Physalis mosaic virus only reached 4cm below the surface.

The researchers hypothesize that nanoparticle geometry and surface chemistry could play a role in how it moves through the soil.

For example, having a tubular structure might partly explain why Tobacco mild green mosaic virus travels farther than most of the other nanoparticles that are spherical in shape.

Also, its surface chemistry is naturally more diverse than synthetic particles like PLGA and silica, which could cause it to interact with the soil differently.

While these design rules may apply to Tobacco mild green mosaic virus, the researchers say more work is needed to better understand why other nanoparticles behave the way they do.

“We’re taking concepts we’ve learned from nanomedicine, where we’re developing nanoparticles for targeted drug delivery, and applying them to agriculture,” Steinmetz said.

“In the medical setting, we also see that nanocarriers with skinny, tubular shapes and diverse surface chemistries can navigate the body better. It makes sense that a plant virus can more easily penetrate and move through the soil–probably because that’s where it naturally resides.”

In terms of safety, Tobacco mild green mosaic virus can infect plants of the Solanaceae (or nightshade) family like tomatoes, potatoes and eggplants, but is benign to thousands of other plant species.

Also, the virus is only transmitted by mechanical contact between two plants, not through the air.

That means if one field is being treated with this virus, nearby fields would not be at risk for contamination, researchers said.

Modelling pesticide delivery

The team also developed a computational model that can be used to predict how different pesticide nanocarriers behave in the soil–how deep they can travel; how much of them need to be applied to the soil; and how long they will take to release their load of pesticide.

“Researchers working with a different plant virus or nanomaterial could use our model to determine how well their particle would work as a pesticide delivery agent,” UC San Diego bioengineering PhD student in Steinmetz’s lab and first author Paul Chariou said.

“It also cuts down on experimental workload. Testing just one nanoparticle for this study involves running hundreds of assays, collecting all the fractions from each column and analysing them,” he said.

“This all takes at least one month. But with the model, it only took us about 10 soil columns and four days to test a new nanoparticle.”

As a next step, Steinmetz and her team are testing Tobacco mild green mosaic virus nanoparticles with pesticide loads. The goal is to test them in the field in the near future.

Source: University of California – San Diego

Paper title: “Soil mobility of synthetic and virus-based model nanoparticles.” Co-authors include Alan B. Dogan, Alexandra G. Welsh, Gerald M. Saidel and Harihara Baskaran, Case Western Reserve University.

 

https://www.eurekalert.org/emb_releases/2019-05/uoc–hpv051719.ph