During a Wagga Wagga field day, NSW DPI researchers gave an overview of the new Septoria projects and led a discussion on variety resistance levels and their impact on disease development. Photo: Andrew Milgate, NSW DPI.

THE GRAINS Research and Development Corporation has announced a series of investments to reduce the impact of the wheat disease Septoria tritici blotch (STB).

STB is a persistent issue for wheat growers in the high and medium rainfall zones of the northern and southern grain growing regions.

If left unmanaged, STB can reduce yields by up to 50 percent.

Traditional control by fungicides is estimated to cost the industry $121 million per year, and resistance to some common fungicides including triazoles and strobilurin used to control STB is evolving.

Together, the NSW Department of Primary Industries, the Australian National University and GRDC are investing $8M over five years to identify novel STB resistance genes and incorporate them into new Australian wheat varieties.

GRDC genetic technologies officer Prameela Vanambathina said the investments will approach the STB problem from three different angles.

“We are seeking to identify novel resistance genes, optimal combinations of adult plant resistance genes, and understand plant pathogen interactions,” Ms Vanambathina said.

“We hope these three investments will provide tools and knowledge essential to reduce the impact of the disease for Australian grain growers.

“Most wheat varieties are susceptible to STB, leading to increased use of fungicides to control the disease.

“There’s a growing fungicide resistance problem, and the identification of new seed sources resistant to Australian pathotypes is crucial.”

Septoria tritici blotch. Photo: Grant Hollaway/GRDC

The first of the three projects aims to discover and transfer novel adult plant resistance genes for STB resistance into wheat-breeding programs.

The project will be led by Andrew Milgate of NSW DPI and aims to identify novel genetic resources that are resistant to STB under Australian environments.

The investment’s objectives include identifying and characterising novel sources of adult plant resistance from international and Australian germplasm pools, validating these genes and transferring the genetic potential into elite Australian wheat lines.

“Previous GRDC investment with NSW DPI and ANU has already identified genes that can contribute to adult plant resistance to STB,” Ms Vanambathina said.

Testing genes

The focus of the second project is on testing optimal combinations of these genes to identify the best ones, and a smaller number of genes for stable adult plant resistance.

Dr Milgate said this partnership will see different combinations of resistance genes, previously identified by NSW DPI researchers in Wagga Wagga, to be bred into wheat varieties.

Combining these high-quality genes together provides added, more stable protection and ensures the genes continue to be effective against STB, which can evolve new virulence rapidly.

“For breeders to efficiently use the resistance genes we also need to provide them with molecular makers that can be used to track the genes in their breeding programs,” Dr Milgate said.

Having molecular markers makes it much faster and more accurate to bred wheat with the desirable resistance genes, meaning new wheat with improved STB resistance can be delivered to growers sooner.

“This is another important component of the projects: to find where the genes are located on the wheat genome and what DNA sequence changes can be used as markers.”

Understanding STB causes

ANU Professor Peter Solomon said ANU was pleased to continue its long-standing partnership with GRDC to tackle diseases that affect the viability and productivity of wheat in the third STB project.

“Despite the impact that STB has on growers, disease and genetic resistance remains poorly understood,” Professor Solomon said.

“This investment by GRDC will enable us to work with colleagues at Birmingham University in the UK to dissect the interaction between key pathogen proteins responsible for virulence and their corresponding host resistance genes in progressing disease.

“The outcomes will significantly advance our understanding of how the fungus Zymoseptoria tritici causes STB. The data generated will be used to develop an approach for screening disease-resistant cultivars.”

Source: GRDC